RPGRIP1L Polyklonaler Antikörper

RPGRIP1L Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 29778-1-AP

Synonyme

CORS3, DKFZp686C0668, FTM, JBTS7, KIAA1005, MKS5, Protein fantom, RPGRIP1 like, RPGRIP1 like protein, RPGRIP1L

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  WB analysis using 29778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29778-1-AP (RPGRIP1L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29778-1-AP (RPGRIP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 29778-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 29778-1-AP (RPGRIP1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

29778-1-AP bindet in WB, IF, ELISA RPGRIP1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPGRIP1L fusion protein Ag31168
• Vollständiger Name: RPGRIP1-like
• Berechnetes Molekulargewicht: 151 kDa
• Beobachtetes Molekulargewicht: 151 kDa
• GenBank-Zugangsnummer: NM_015272
• Gene symbol: RPGRIP1L
• Gene ID (NCBI): 23322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5).

Publikationen

Species	Application	Title
human	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human,zebrafish	WB	Dis Model Mech; Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants; Authors - Jun Wang
	IF	bioRxiv; Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis; Authors - Manu Ahmed
mouse	WB	Cell Mol Neurobiol; Downregulation of Hmox1 and Rpgrip1l Expression Linked to Risk-Taking Behavior, Reduced Depressive Symptoms, and Diminished Novelty Socialization in SUMO1 Knockout Mice; Authors - Qiwei Dai