RPH3A Polyklonaler Antikörper

RPH3A Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11396-1-AP

Synonyme

Exophilin 1, KIAA0985, Rabphilin 3A, rabphilin 3A homolog (mouse), RPH3A

---

Galerie der Validierungsdaten

• 

  WB analysis of COLO 320 using 11396-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11396-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 11396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of COLO 320 using 11396-1-AP

  Immunofluorescent analysis of COLO 320 cells using 11396-1-AP (RPH3A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

11396-1-AP bindet in WB, IHC, IF, ELISA RPH3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPH3A fusion protein Ag1956
• Vollständiger Name: rabphilin 3A homolog (mouse)
• Berechnetes Molekulargewicht: 690 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC017259
• Gene symbol: RPH3A
• Gene ID (NCBI): 22895
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells; Authors - Tatsuaki Kurosaki
mouse	WB	Front Aging Neurosci; Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses; Authors - Elena Ferrari
mouse	WB,IF	Pharmacol Res; Rabphilin-3A as a novel target to reverse α-synuclein-induced synaptic loss in Parkinson's disease; Authors - Elena Ferrari
rat	WB	Elife; Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis; Authors - Tianzhi Li