RPH3A Polyklonaler Antikörper

RPH3A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11396-1-PBS

Synonyme

Rabphilin 3A, Rabphilin, KIAA0985, Exophilin-1, Exophilin1

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  WB analysis of COLO 320 using 11396-1-AP (same clone as 11396-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11396-1-AP (same clone as 11396-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11396-1-AP (RPH3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11396-1-AP (same clone as 11396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11396-1-AP (same clone as 11396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11396-1-AP (RPH3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 11396-1-AP (same clone as 11396-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using RPH3A antibody (11396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11396-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11396-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RPH3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPH3A fusion protein Ag1956
• Vollständiger Name: rabphilin 3A homolog (mouse)
• Berechnetes Molekulargewicht: 690 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC017259
• Gene symbol: RPH3A
• Gene ID (NCBI): 22895
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.