RPL13 Rekombinanter Antikörper

RPL13 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250752D2

Kat-Nr. : 86205-2-RR

Synonyme

60S ribosomal protein L13, BBC1, D16S444E, Large ribosomal subunit protein eL13, OK/SW-cl.46

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  WB analysis using 86205-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86205-2-RR (RPL13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86205-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL13 antibody (86205-2-RR, Clone: 250752D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86205-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86205-2-RR against Human RPL13 were performed. The affinity constant is 0.10 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COS-7-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86205-2-RR bindet in WB, IF/ICC, ELISA RPL13 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RPL13 fusion protein Ag1814
• Vollständiger Name: ribosomal protein L13
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC014167
• Gene symbol: RPL13
• Gene ID (NCBI): 6137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL13 may be a plausible candidate tumor suppressor gene, as knocking down L13 expression using small interfering RNA (siRNA) resulted in drastic attenuation of cancer cell growth with significant G1 and G2/M arrest of the cell cycle[PMID:16786168].