RPL13A Polyklonaler Antikörper

RPL13A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14633-1-AP

Synonyme

ribosomal protein L13a, Large ribosomal subunit protein uL13

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  WB analysis using 14633-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14633-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14633-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 14633-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14633-1-AP (RPL13A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, RAW264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14633-1-AP bindet in WB, IHC, ELISA RPL13A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL13A fusion protein Ag6212
• Vollständiger Name: ribosomal protein L13a
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC065236
• Gene symbol: RPL13A
• Gene ID (NCBI): 23521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein L13a(RPL13a) is a member of the L13 ribosomal protein family and a structural component of the large 60S ribosomal subunit. It's a reference gene(RG) that stably expressed not only during differentiation, but also during expansion to higher passages. And it's suggested as a RG for a variety research field. RPL13a is a key component of the IFN-gamma-activated inhibitor of translation(GAIT) complex, which inhibits translation of a select group of inflammation-related transcripts via binding to rhe 3' UTR of target mRNA.once phosphorylated of Ser77 by ZIPK, RPL13a released from the complex.