RPL15 Rekombinanter Antikörper

RPL15 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242029E3

Kat-Nr. : 84900-1-RR

Synonyme

242029E3, 60S ribosomal protein L15, EC45, Large ribosomal subunit protein eL15, ribosomal protein L15

---

• 

  WB analysis using 84900-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84900-1-RR (RPL15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse colon using 84900-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84900-1-RR (RPL15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 84900-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84900-1-RR (RPL15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HepG2-Zellen, K-562-Zellen, Maus-Kolongewebe, NCI-H1299-Zellen, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84900-1-RR bindet in WB, IHC, ELISA RPL15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RPL15 fusion protein Ag9948
• Vollständiger Name: ribosomal protein L15
• Berechnetes Molekulargewicht: 204 aa, 24 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC070328
• Gene symbol: RPL15
• Gene ID (NCBI): 6138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL15 (ribosomal protein L15) is a component of the ribosomal large subunit 60S, participating in the assembly process of ribosomal subunits and involved in the processing of rRNA. Moreover, RPL15 deletion will lead to abnormalities in the biogenesis of ribosomal subunits. The abnormal expression or mutation of RPL15 protein is also closely related to the occurrence and development of many diseases. RPL15 was highly expressed in gastric cancer cell lines, and interference with the expression of RPL15 could inhibit gastric cancer cell growth. In addition, RPL15 in skin squamous cell carcinoma tissues and pancreatic cancer cell lines decreased sharply, which had a great relationship with the survival rate. PMID:35410346