RPL17 Monoklonaler Antikörper

RPL17 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C5E6

Kat-Nr. : 67223-1-PBS

Synonyme

1C5E6, Large ribosomal subunit protein uL22, PD-1, uL22

---

• 

  WB analysis using 67223-1-Ig (same clone as 67223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67223-1-Ig (RPL17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67223-1-Ig (RPL17 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL17 antibody (67223-1-Ig, Clone: 1C5E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67223-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RPL17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPL17 fusion protein Ag5393
• Vollständiger Name: ribosomal protein L17
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC066323
• Gene symbol: RPL17
• Gene ID (NCBI): 6139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.