RPL18A Polyklonaler Antikörper

RPL18A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14653-1-PBS

Synonyme

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  WB analysis of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14653-1-AP (same clone as 14653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 14653-1-AP (same clone as 14653-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 14653-1-AP (same clone as 14653-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 14653-1-AP (same clone as 14653-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL18A antibody (14653-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14653-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RPL18A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL18A fusion protein Ag6251
• Vollständiger Name: ribosomal protein L18a
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC066319
• Gene symbol: RPL18A
• Gene ID (NCBI): 6142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.