RPL23 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPL23 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 16086-1-AP

Synonyme

60S ribosomal protein L17, 60S ribosomal protein L23, ribosomal protein L23, rpL17, RPL23

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  WB analysis using 16086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16086-1-AP (RPL23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16086-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16086-1-AP (RPL23 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16086-1-AP

  IP result of anti-RPL23 (IP:16086-1-AP, 4ug; Detection:16086-1-AP 1:3000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP (RPL23 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP (RPL23 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16086-1-AP (RPL23 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16086-1-AP (RPL23 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• RIP: See 1 publications below

Produktinformation

16086-1-AP bindet in WB, IHC, IF, IP, RIP, ELISA RPL23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL23 fusion protein Ag9120
• Vollständiger Name: ribosomal protein L23
• Berechnetes Molekulargewicht: 140 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC010114
• Gene symbol: RPL23
• Gene ID (NCBI): 9349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.; Authors - Kaloyan M Tsanov
human,mouse	IHC	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
human	WB	Blood; Defective ribosome assembly in Shwachman-Diamond syndrome.; Authors - Wong Chi C CC
human	WB	Breast Cancer Res; U2AF2-SNORA68 promotes triple-negative breast cancer stemness through the translocation of RPL23 from nucleoplasm to nucleolus and c-Myc expression; Authors - Wenrong Zhang
mouse	WB,IHC	Sci Rep; Ribosomal protein L23 negatively regulates cellular apoptosis via the RPL23/Miz-1/c-Myc circuit in higher-risk myelodysplastic syndrome.; Authors - Yuekun Qi ; KD Validated
human	WB,IHC,IF,RIP	Front Oncol; Ribosomal Protein L23 Drives the Metastasis of Hepatocellular Carcinoma via Upregulating MMP9.; Authors - Minli Yang ; KD Validated