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  • KD/KO Validated

RPL24 Polyklonaler Antikörper

RPL24 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 17082-1-AP

Synonyme

ribosomal protein L24, Large ribosomal subunit protein eL24, 60S ribosomal protein L30, 60S ribosomal protein L24



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HEK-293-Zellen, Jurkat-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Plazenta-Gewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Milzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17082-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA RPL24 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus, Krallenfrosch (Xenopus)
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RPL24 fusion protein Ag7085
Vollständiger Name ribosomal protein L24
Berechnetes Molekulargewicht 18 kDa
Beobachtetes Molekulargewicht 21-23 kDa
GenBank-ZugangsnummerBC000690
Gene symbol RPL24
Gene ID (NCBI) 6152
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688]

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for RPL24 antibody 17082-1-APProtokoll herunterladen
IHC protocol for RPL24 antibody 17082-1-APProtokoll herunterladenl
IF protocol for RPL24 antibody 17082-1-APProtokoll herunterladen
IP protocol for RPL24 antibody 17082-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell

Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.

Authors - Sridevi Challa
  • KD Validated
humanWB

Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Authors - Kaosheng Lv
IF

Neuron

Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.

Authors - Roberta Cagnetta
WB

Dev Cell

Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions

Authors - Max Koppers
humanIHC, WB

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
WB,IP

Cell Mol Life Sci

Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner

Authors - Yonat Tzur

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Erica (Verified Customer) (05-15-2019)

The antibody works very well for WB in both Human HEK and MEF cells. I used 1:2000 dilution in 2%BSA, and it always work well. It doesn't work very well for IP though..

  • Applications: Western Blot, Immunoprecipitation,
  • Cell Tissue Type: Mammalian cells