RPL31 Polyklonaler Antikörper

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• KD/KO Validated

RPL31 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 16497-1-AP

Synonyme

ribosomal protein L31, Large ribosomal subunit protein eL31, 60S ribosomal protein L31

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  WB analysis of HepG2 using 16497-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16497-1-AP

  WB result of RPL31 antibody (16497-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL31 transfected HepG2 cells.

• 

  WB analysis of NIH/3T3 using 16497-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16497-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16497-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16497-1-AP (RPL31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

16497-1-AP bindet in WB, IF/ICC, ELISA RPL31 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL31 fusion protein Ag9648
• Vollständiger Name: ribosomal protein L31
• Berechnetes Molekulargewicht: 125 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC017343
• Gene symbol: RPL31
• Gene ID (NCBI): 6160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. Ribosomal protein L31 gene is a component of the 60S large ribosomal subunit encoded by RPL31 gene, while ribosomal protein L31 (RPL31) is an important constituent of peptidyltransferase center [PMID:22714919].

Publikationen

Species	Application	Title
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
mouse	IF	J Med Genet; Bi-allelic variants in chromatoid body protein TDRD6 cause spermiogenesis defects and severe oligoasthenoteratozoospermia in humans; Authors - Rui Guo
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Weak band around 15kDa

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart