Ribosomal protein L4 Monoklonaler Antikörper

Ribosomal protein L4 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

3C7A8

Kat-Nr. : 67028-1-Ig

Synonyme

RPL4, 3C7A8, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4

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  WB analysis using 67028-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67028-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 67028-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 67028-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67028-1-Ig (Ribosomal protein L4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67028-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 67028-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (67028-1-Ig, Clone: 3C7A8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 67028-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (67028-1-Ig, Clone:3C7A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

67028-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA Ribosomal protein L4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ribosomal protein L4 fusion protein Ag28728
• Vollständiger Name: ribosomal protein L4
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC009888
• Gene symbol: Ribosomal protein L4
• Gene ID (NCBI): 6124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Publikationen

Species	Application	Title
human	WB,IP	Nat Commun; The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.; Authors - Matthias M Zimmer
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
mouse	WB	Cell Rep; FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.; Authors - Sneha Shah
human	WB,IP,CoIP	Exp Cell Res; PRC1 promotes ovarian cancer progression by binding to RPL4 and increasing MDM2-mediated p53 ubiquitination; Authors - Yinyin Xu
rat	WB	Free Radic Biol Med; Mitochondrial Electron Transfer Chain Activation in Atrophic Muscle: Implications for Calcium Signaling and Chondrocyte Apoptosis in Osteoarthritis; Authors - Ailin Li