RPL6 Monoklonaler Antikörper

RPL6 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3F6F8

Kat-Nr. : 67729-1-Ig

Synonyme

3F6F8, 60S ribosomal protein L6, Large ribosomal subunit protein eL6, Neoplasm related protein C140, Neoplasm-related protein C140

---

• 

  WB analysis using 67729-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67729-1-Ig (RPL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of L02 using 67729-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using RPL6 antibody (67729-1-Ig, Clone: 3F6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67729-1-Ig bindet in WB, IF/ICC, ELISA RPL6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPL6 fusion protein Ag6840
• Vollständiger Name: ribosomal protein L6
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC004138
• Gene symbol: RPL6
• Gene ID (NCBI): 6128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].