RPLP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPLP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 21636-1-AP

Synonyme

P1, ribosomal protein, large, P1, RPLP1, RPP1, RRP1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 21636-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21636-1-AP (RPLP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21636-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21636-1-AP (RPLP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21636-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21636-1-AP (RPLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21636-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21636-1-AP (RPLP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 21636-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21636-1-AP (RPLP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 21636-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21636-1-AP (RPLP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 21636-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21636-1-AP (RPLP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 21636-1-AP

  Immunofluorescent analysis of HepG2 cells using 21636-1-AP (RPLP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

21636-1-AP bindet in WB, IHC, IF, ELISA RPLP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPLP1 fusion protein Ag16268
• Vollständiger Name: ribosomal protein, large, P1
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC003369
• Gene symbol: RPLP1
• Gene ID (NCBI): 6176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

60S acidic ribosomal protein P1 (RRP1) is a 114 amino acid protein, which belongs to the ribosomal protein L12P family. RRP1 plays an important role in the elongation step of protein synthesis. The molecular weight of modified RRP1 is 15-20 kDa.

Publikationen

Species	Application	Title
pig	WB	Virulence; RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome.; Authors - Longxiang Zhang ; KD Validated
mouse	WB	Mol Hum Reprod; 60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro.; Authors - Zahraa Alali
human	WB	Int J Mol Sci; RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro; Authors - Riley Peterson
human	WB,IHC	Am J Cancer Res; Unveiling the prognostic implications of RPLP1 upregulation in osteosarcoma; Authors - Xinhui Du