Ribophorin I Rekombinanter Antikörper

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• KD/KO Validated

Ribophorin I Rekombinant Antikörper für WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240044D4

Kat-Nr. : 83250-2-PBS

Synonyme

RPN1, 240044D4, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Ribophorin-1

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  WB analysis using 83250-2-RR (same clone as 83250-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83250-2-RR (RPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83250-2-RR (same clone as 83250-2-PBS)

  WB result of Ribophorin I antibody (83250-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribophorin I transfected HepG2 cells. This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00231-3

  Sandwich ELISA standard curve of MP00231-3, Human Ribophorin I Recombinant Matched Antibody Pair - PBS only. 83250-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3576. 83250-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00231-3

  The mean Ribophorin I concentration was determined to be 145.4 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 11.0 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load.

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  IHC staining of human stomach cancer using 83250-2-RR (same clone as 83250-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83250-2-RR (Ribophorin I antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83250-2-RR (same clone as 83250-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPN1 Recombinant antibody (83250-2-RR, Clone:240044D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83250-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83250-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83250-2-PBS against Human Ribophorin I was performed. The affinity constant is 1.69 nM.

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  Affinity and Kinetic Characterization of 83250-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83250-2-RR against Human RPN1 were performed. The affinity constant is 0.154 uM.

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Geprüfte Anwendungen

Produktinformation

83250-2-PBS bindet in WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Ribophorin I und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ribophorin I fusion protein Ag3576
• Vollständiger Name: ribophorin I
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 66-69 kDa
• GenBank-Zugangsnummer: BC010839
• Gene symbol: RPN1
• Gene ID (NCBI): 6184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.