RPS12 Monoklonaler Antikörper

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• KD/KO Validated

RPS12 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A3D1

Kat-Nr. : 67683-1-PBS

Synonyme

1A3D1, 40S ribosomal protein S12, ribosomal protein S12, Small ribosomal subunit protein eS12

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  WB analysis using 67683-1-Ig (same clone as 67683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

  WB result of RPS12 antibody (67683-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS12 transfected HepG2 cells. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 67683-1-Ig (same clone as 67683-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67683-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RPS12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPS12 fusion protein Ag9649
• Vollständiger Name: ribosomal protein S12
• Berechnetes Molekulargewicht: 132 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC017321
• Gene symbol: RPS12
• Gene ID (NCBI): 6206
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.