RPS17 Rekombinanter Antikörper

RPS17 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240851H3

Kat-Nr. : 83848-4-PBS

Synonyme

240851H3, 40S ribosomal protein S17, DBA4, ribosomal protein S17, RPS17L

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  WB analysis using 83848-4-RR (same clone as 83848-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83848-4-RR (same clone as 83848-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83848-4-RR (same clone as 83848-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83848-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

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Geprüfte Anwendungen

Produktinformation

83848-4-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA RPS17 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RPS17 fusion protein Ag9334
• Vollständiger Name: ribosomal protein S17
• Berechnetes Molekulargewicht: 135 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC009407
• Gene symbol: RPS17
• Gene ID (NCBI): 6218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.