RPS20 Monoklonaler Antikörper

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• KD/KO Validated

RPS20 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E9B4

Kat-Nr. : 68270-1-PBS

Synonyme

3E9B4, 40S ribosomal protein S20, ribosomal protein S20, Small ribosomal subunit protein uS10

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  WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68270-1-Ig (same clone as 68270-1-PBS)

  WB result of RPS20 antibody (68270-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS20 transfected HepG2 cells. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50774-1

  Cytometric bead array standard curve of MP50774-1, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-2-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP50774-2

  Cytometric bead array standard curve of MP50774-2, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-3-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 3.125-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68270-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA RPS20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPS20 fusion protein Ag8275
• Vollständiger Name: ribosomal protein S20
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC007507
• Gene symbol: RPS20
• Gene ID (NCBI): 6224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.