RPS25 Polyklonaler Antikörper

RPS25 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 23599-1-AP

Synonyme

40S ribosomal protein S25, ribosomal protein S25, RPS25

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  WB analysis using 23599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23599-1-AP (RPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23599-1-AP (RPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23599-1-AP (RPS25 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23599-1-AP (RPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23599-1-AP (RPS25 Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 23599-1-AP

  Immunofluorescent analysis of MCF-7 cells using 23599-1-AP (RPS25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

23599-1-AP bindet in WB, IHC, IF/ICC, ELISA RPS25 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS25 fusion protein Ag7890
• Vollständiger Name: ribosomal protein S25
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC004986
• Gene symbol: RPS25
• Gene ID (NCBI): 6230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting.

Publikationen

Species	Application	Title
human	WB	ACS Nano; Platelet-Derived Apoptotic Vesicles Promote Bone Regeneration via Golgi Phosphoprotein 2 (GOLPH2)-AKT Signaling Axis; Authors - Yuhe Jiang
human	WB	Mol Cell Proteomics; Identification and characterization of potential biomarkers by quantitative tissue proteomics of primary lung adenocarcinoma.; Authors - Chiung-Hung Hsu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (07-09-2020)

This antibody works well for screening of crispr knock-in. There were a couple non-specific bands above though.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

RPS25 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:23599-1-AP)