RPS27A Polyklonaler Antikörper

RPS27A Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14946-1-PBS

Synonyme

---

• 

  WB analysis of mouse cerebellum using 14946-1-AP (same clone as 14946-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14946-1-AP (RPS27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14946-1-AP (same clone as 14946-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14946-1-AP (RPS27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 14946-1-AP (same clone as 14946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 14946-1-AP (same clone as 14946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 14946-1-AP (same clone as 14946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 14946-1-AP (same clone as 14946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 14946-1-AP (same clone as 14946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14946-1-AP (same clone as 14946-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS27A antibody (14946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 14946-1-AP (same clone as 14946-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RPS27A (14946-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

14946-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA RPS27A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS27A fusion protein Ag6750
• Vollständiger Name: ribosomal protein S27a
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 9 kDa, 18 kDa
• GenBank-Zugangsnummer: BC001392
• Gene symbol: RPS27A
• Gene ID (NCBI): 6233
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]