RPS3 Polyklonaler Antikörper

RPS3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15198-1-AP

Synonyme

ribosomal protein S3, OK/SW-cl.26, FLJ27450, FLJ26283, EC:4.2.99.18

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  WB analysis of mouse brain using 15198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 15198-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15198-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15198-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15198-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15198-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15198-1-AP

  IP result of anti-RPS3 (IP:15198-1-AP, 4ug; Detection:15198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 15198-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS3 antibody (15198-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, COLO 320-Zellen, HeLa-Zellen, Maus-Kolongewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15198-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RPS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Hefe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS3 fusion protein Ag7346
• Vollständiger Name: ribosomal protein S3
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC003137
• Gene symbol: RPS3
• Gene ID (NCBI): 6188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.

Publikationen

Species	Application	Title
yeast	WB	PLoS Genet; Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes.; Authors - Faza Marius B MB