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RPS3 Rekombinanter Antikörper

RPS3 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242504F3

Kat-Nr. : 84947-4-PBS

Synonyme

242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450

Filter:
  • Western Blot (WB) analysis of various lysates using RPS3 Recombinant antibody (84947-4-RR)

    WB analysis using 84947-4-RR (same clone as 84947-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84947-4-RR (RPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS3 Recombinant antibody (84947-4-RR)

    IHC staining of human colon cancer using 84947-4-RR (same clone as 84947-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84947-4-RR (RPS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RPS3 Recombinant antibody (84947-4-RR)

    IF Staining of NIH/3T3 using 84947-4-RR (same clone as 84947-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RPS3 antibody (84947-4-RR, Clone: 242504F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84947-4-RR

    Affinity and Kinetic Characterization of 84947-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84947-4-RR against Human RPS3 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84947-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RPS3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RPS3 fusion protein Ag7346
Vollständiger Name ribosomal protein S3
Berechnetes Molekulargewicht 27 kDa
Beobachtetes Molekulargewicht 30 kDa
GenBank-ZugangsnummerBC003137
Gene symbol RPS3
Gene ID (NCBI) 6188
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.