RPS7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPS7 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 14491-1-AP

Synonyme

40S ribosomal protein S7, DBA8, ribosomal protein S7, RPS7

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 14491-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14491-1-AP (RPS7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14491-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14491-1-AP (RPS7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 14491-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14491-1-AP (RPS7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 14491-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14491-1-AP (RPS7 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14491-1-AP bindet in WB, IHC, IF, ELISA RPS7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS7 fusion protein Ag5892
• Vollständiger Name: ribosomal protein S7
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC061901
• Gene symbol: RPS7
• Gene ID (NCBI): 6201
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S7(RPS7) belongs to the RPs, which is structural compoent of the ribosome invilved in protein synthesis. It interacts with and inhibit mdm2 mediated p53 degradation function. Also RPS7 has a essential role in early development. RPS7 involves in the maturation of ribosomal RNAs in the large or the small ribosomal subunit production pathway

Publikationen

Species	Application	Title
mouse	WB	Nat Chem Biol; Identifying the functional contribution of the defatty-acylase activity of SIRT6.; Authors - Xiaoyu Zhang
human	WB	Sci Rep; Stabilization of SIRT7 deacetylase by viral oncoprotein HBx leads to inhibition of growth restrictive RPS7 gene and facilitates cellular transformation.; Authors - Vijaya Pandey
human	WB	Exp Cell Res; Combination of DESI2 and endostatin gene therapy significantly improves antitumor efficacy by accumulating DNA lesions, inducing apoptosis and inhibiting angiogenesis.; Authors - Huaying Yan
human	WB	Cell Death Dis; SMYD2 promotes tumorigenesis and metastasis of lung adenocarcinoma through RPS7.; Authors - Lei Wu
mouse	WB,IHC,IF	Biomed Res Int; Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury; Authors - Hao Zhang ; KD Validated
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (07-09-2020)

This antibody has so many non-specific bands. Considering the fact that ribosomal proteins are so abundant, having these many non-specific bands is not usual for an r-protein antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

RPS7 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:14491-1-AP)