RPS8 Polyklonaler Antikörper

RPS8 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 18228-1-AP

Synonyme

Small ribosomal subunit protein eS8, ribosomal protein S8, OK/SW-cl.83, 40S ribosomal protein S8

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  WB analysis using 18228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 18228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 18228-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18228-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 18228-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 18228-1-AP

  Immunofluorescent analysis of A375 cells using 18228-1-AP (RPS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, Mauslebergewebe, Maus-Pankreasgewebe, PC-3-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

18228-1-AP bindet in WB, IHC, IF/ICC, ELISA RPS8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS8 fusion protein Ag12313
• Vollständiger Name: ribosomal protein S8
• Berechnetes Molekulargewicht: 208 aa, 24 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC070875
• Gene symbol: RPS8
• Gene ID (NCBI): 6202
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Nat Cell Biol; Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties; Authors - Dennis K Jeppesen
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	IHC	Cancer Biol Med; A novel recurrence-associated metabolic prognostic model for risk stratification and therapeutic response prediction in patients with stage I lung adenocarcinoma.; Authors - Chengming Liu
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	IHC	Lab Invest; Proteins associated with pancreatic cancer survival in patients with resectable pancreatic ductal adenocarcinoma.; Authors - Ru Chen
human	IHC	Oncol Rep; Identification of 40S ribosomal protein S8 as a novel biomarker for alcohol‑associated hepatocellular carcinoma using weighted gene co‑expression network analysis.; Authors - Ningrui Bi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anita (Verified Customer) (10-30-2020)

Works at 1:1000 dilution for HeLa cells and C. elegans nematodes

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans and HeLa cells

RPS8 Antibody Western Blot validation (1:1000 dilution) in C. elegans and HeLa cells (Cat no:18228-1-AP)