RPS9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPS9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 18215-1-AP

Synonyme

40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4

---

• 

  WB analysis of mouse uterus using 18215-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 18215-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPS9 antibody (18215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Uterusgewebe, HeLa-Zellen, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hodengewebe, Mauslungengewebe, Maus-Pankreasgewebe, Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18215-1-AP bindet in WB, IHC, IF/ICC, ELISA RPS9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS9 fusion protein Ag12945
• Vollständiger Name: ribosomal protein S9
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC000802
• Gene symbol: RPS9
• Gene ID (NCBI): 6203
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Proc Natl Acad Sci U S A; Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.; Authors - Elizabeth I Vink ; KD Validated
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	Sci Rep; Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.; Authors - Haiping Tang
human	WB	Front Immunol; Gradient Boosting Machine Learning Model for Defective Endometrial Receptivity Prediction by Macrophage-Endometrium Interaction Modules.; Authors - Bohan Li
human	WB	Genes (Basel); Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.; Authors - Meijuan Zou ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Wei (Verified Customer) (01-30-2020)

Single, strong band as predicted size 25kDa

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart