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RPUSD1 Polyklonaler Antikörper

RPUSD1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25578-1-PBS

Synonyme

RLUCL, C16orf40

Filter:
  • Western Blot (WB) analysis of various lysates using RPUSD1 Polyclonal antibody (25578-1-AP)

    WB analysis using 25578-1-AP (same clone as 25578-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25578-1-AP (RPUSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPUSD1 Polyclonal antibody (25578-1-AP)

    IP experiment of HeLa using 25578-1-AP (same clone as 25578-1-PBS)

    IP result of anti-RPUSD1 (IP:25578-1-AP, 4ug; Detection:25578-1-AP 1:500) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPUSD1 Polyclonal antibody (25578-1-AP)

    IF Staining of HeLa using 25578-1-AP (same clone as 25578-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPUSD1 antibody (25578-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25578-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25578-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA RPUSD1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RPUSD1 fusion protein Ag22314
Vollständiger Name RNA pseudouridylate synthase domain containing 1
Berechnetes Molekulargewicht 312 aa, 35 kDa
Beobachtetes Molekulargewicht34~35 kDa
GenBank-ZugangsnummerBC011783
Gene symbol RPUSD1
Gene ID (NCBI) 113000
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPUSD1 (RNA pseudouridylate synthase domain-containing protein 1) also known as RLUCL (Ribosomal large subunit pseudouridine synthase C-like protein), was significantly upregulated in HCC and expressed at higher levels in advanced stage or poorly differentiated HCC tissues (PMID: 36437949).