RRAGB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RRAGB Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 13023-1-AP

Synonyme

EC:3.6.5.-, Rag B, RagB, Ras related GTP binding B, Ras related GTP binding protein B

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  WB analysis using 13023-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13023-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13023-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13023-1-AP

  IP result of anti-RRAGB (IP:13023-1-AP, 4ug; Detection:13023-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe, HeLa-Zellen, humanes Hirngewebe, humanes Hodengewebe, Jurkat-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

13023-1-AP bindet in WB, IHC, IF, IP, RIP, ELISA RRAGB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRAGB fusion protein Ag4116
• Vollständiger Name: Ras-related GTP binding B
• Berechnetes Molekulargewicht: 346 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC034726
• Gene symbol: RRAGB
• Gene ID (NCBI): 10325
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,RIP,IHC	Mol Cancer; circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.; Authors - Xiaomin Li
mouse	WB,IF	J Clin Invest; YAP plays a crucial role in the development of cardiomyopathy in lysosomal storage diseases.; Authors - Shohei Ikeda ; KO Validated
mouse	WB	PLoS One; Ursolic Acid Inhibits Leucine-Stimulated mTORC1 Signaling by Suppressing mTOR Localization to Lysosome.; Authors - Xiang Ou
rat	WB	Physiol Rep; Tissue-specific expression differences in Ras-related GTP-binding proteins in male rats; Authors - Gregory N Kincheloe
mouse	WB	Cell Signal; CircMRP4 orchestrates podocytes injury via the miR-499-5p/RRAGB/mTORC1 axis in diabetic kidney disease; Authors - Shujun Deng
human	WB,IP	Cell Metab; Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism; Authors - Ning Wang