RRAGC Polyklonaler Antikörper

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• KD/KO Validated

RRAGC Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 26989-1-AP

Synonyme

EC:3.6.5.-, GTPase interacting protein 2, GTPase-interacting protein 2, GTR2, Rag C

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  WB analysis using 26989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26989-1-AP

  WB result of RRAGC antibody (26989-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRAGC transfected Jurkat cells.

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  WB analysis of NIH/3T3 using 26989-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26989-1-AP (RRAGC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

26989-1-AP bindet in WB, IHC, IP, ELISA RRAGC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRAGC fusion protein Ag25780
• Vollständiger Name: Ras-related GTP binding C
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC016668
• Gene symbol: RRAGC
• Gene ID (NCBI): 64121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RRAGC (Ras-related GTP-binding protein C) is a small monomeric GTPase that functions as part of the mTORC1 nutrient-sensing pathway. Together with its dimerization partners RRAGA or RRAGB, RRAGC localizes mainly to the cytoplasm and, in response to amino-acid availability, recruits the mTORC1 complex to the lysosomal surface where mTORC1 can be activated by Rheb. This makes RRAGC a key molecular switch linking cellular metabolic state to growth control.

Publikationen

Species	Application	Title
human	WB	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	Genet Med; De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood; Authors - Margot R F Reijnders
mouse,human	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li
human	WB,IP	Cell Metab; Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism; Authors - Ning Wang