RRAS Polyklonaler Antikörper

RRAS Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 27457-1-AP

Synonyme

p23, Ras related protein R Ras, RRAS

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Galerie der Validierungsdaten

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  WB analysis using 27457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27457-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27457-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 27457-1-AP

  IP result of anti-RRAS(IP:27457-1-AP, 4ug; Detection:27457-1-AP 1:500) with HT-29 cells lysate 3320 ug.

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  IHC staining of human renal cell carcinoma using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HT-29-Zellen, PC-3-Zellen
• Erfolgreiche IP: HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Gliomgewebe, Maus-Kolongewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

27457-1-AP bindet in WB, IP, IHC, ELISA RRAS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRAS fusion protein Ag26538
• Vollständiger Name: related RAS viral (r-ras) oncogene homolog
• Berechnetes Molekulargewicht: 218 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC016318
• Gene symbol: RRAS
• Gene ID (NCBI): 6237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human，mouse	WB	Biomaterials; Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.; Authors - Yuanyuan Liu
human	WB	Transl Cancer Res; Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses.; Authors - Ruowen Xiao
human	WB	Hepatology; Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis; Authors - Meilin Ma