RRBP1 Rekombinanter Antikörper

RRBP1 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230159G6

Kat-Nr. : 82890-1-RR

Synonyme

180 kDa ribosome receptor homolog, 230159G6, ES/130 related protein, ES/130-related protein, Ribosome-binding protein 1

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  IF Staining of HeLa using 82890-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

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  IF Staining of NIH/3T3 using 82890-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

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  IF Staining of NIH/3T3 using 82890-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

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  FC experiment of U-2 OS using 82890-1-RR

  (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-1-RR, Clone:230159G6) and Alexa Fluor™ Plus 647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 82890-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-1-RR, Clone:230159G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82890-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82890-1-RR against Human RRBP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): U-2 OS, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82890-1-RR bindet in IF/ICC, FC (Intra), ELISA RRBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RRBP1 fusion protein Ag16648
• Vollständiger Name: ribosome binding protein 1 homolog 180kDa (dog)
• Berechnetes Molekulargewicht: 1410 aa, 152 kDa
• Beobachtetes Molekulargewicht: 108 kDa, 152 kDa
• GenBank-Zugangsnummer: BC128577
• Gene symbol: RRBP1
• Gene ID (NCBI): 6238
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)