RREB1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RREB1-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20280-1-AP

Synonyme

RREB1, FINB, HNT, LZ321, Raf-responsive zinc finger protein LZ321

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  WB analysis using 20280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 20280-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20280-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

20280-1-AP bindet in WB, IHC, ChIP, ELISA RREB1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ras responsive element binding protein 1
• Berechnetes Molekulargewicht: 181 kDa
• Beobachtetes Molekulargewicht: 160-181 kDa, 250 kDa
• GenBank-Zugangsnummer: NM_001003698
• Gene symbol: RREB1
• Gene ID (NCBI): 6239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)

Publikationen

Species	Application	Title
human	WB,IHC	Cell Cycle; Knockdown of RREB1 inhibits cell proliferation via enhanced p16 expression in gastric cancer.; Authors - Qi Gao ; KD Validated
human	WB,ChIP	Cell Rep; Oncogenic KRAS effector USP13 promotes metastasis in non-small cell lung cancer through deubiquitinating β-catenin; Authors - Yanguan Guo ; KD Validated