RRM1 Rekombinanter Antikörper

RRM1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250707E6

Kat-Nr. : 86135-1-RR

Synonyme

EC:1.17.4.1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, ribonucleotide reductase M1, RIR1

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  WB analysis using 86135-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86135-1-RR (RRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86135-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RRM1 antibody (86135-1-RR, Clone: 250707E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 86135-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM1 antibody (86135-1-RR, Clone: 250707E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86135-1-RR bindet in WB, IF/ICC, ELISA RRM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RRM1 fusion protein Ag0789
• Vollständiger Name: ribonucleotide reductase M1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC006498
• Gene symbol: RRM1
• Gene ID (NCBI): 6240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival (PMID:17314339).