RRM2 Monoklonaler Antikörper

RRM2 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A9A7

Kat-Nr. : 67006-1-PBS

Synonyme

2A9A7, EC:1.17.4.1, R2, RR2, RR2M

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  WB analysis using 67006-1-Ig (same clone as 67006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 67006-1-Ig (same clone as 67006-1-PBS)

  IP result of anti-RRM2 (IP:67006-1-Ig, 4ug; Detection:67006-1-Ig 1:2000) with HEK-293 cells lysate 1470 ug. This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67006-1-Ig (same clone as 67006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67006-1-Ig (RRM2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67006-1-Ig (same clone as 67006-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM2 antibody (67006-1-Ig, Clone: 2A9A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67006-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67006-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA RRM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RRM2 fusion protein Ag28664
• Vollständiger Name: ribonucleotide reductase M2 polypeptide
• Berechnetes Molekulargewicht: 389 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC030154
• Gene symbol: RRM2
• Gene ID (NCBI): 6241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].