RRN3 Polyklonaler Antikörper

RRN3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25918-1-AP

Synonyme

RNA polymerase I-specific transcription initiation factor RRN3, TIF IA, TIFIA, TIF-IA

---

• 

  WB analysis of HeLa using 25918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25918-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25918-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25918-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25918-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25918-1-AP

  IP result of anti-RRN3 (IP:25918-1-AP, 4ug; Detection:25918-1-AP 1:500) with HeLa cells lysate 3200ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, K-562-Zellen, Maushodengewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

25918-1-AP bindet in WB, IP, chIP, ELISA RRN3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRN3 fusion protein Ag23207
• Vollständiger Name: RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC132688
• Gene symbol: RRN3
• Gene ID (NCBI): 54700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	chIP	Biochem Biophys Res Commun; Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.; Authors - Xiaolei Zhang
	WB	Mol Cell; Control of ribosomal RNA synthesis by hematopoietic transcription factors; Authors - Charles Antony
human	WB	Mol Biol Cell; A role for the kinetochore protein, NUF2, in ribosome biogenesis; Authors - Ty J Brown