RRP1 Polyklonaler Antikörper

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• KD/KO Validated

RRP1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14896-1-AP

Synonyme

Novel nuclear protein 1, NOP52, NNP-1, NNP1, NNP 1

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  WB analysis of HEK-293 using 14896-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14896-1-AP

  WB result of RRP1 antibody (14896-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRP1 transfected HeLa cells.

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  WB analysis of HEK-293 using 14896-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 14896-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14896-1-AP

  Immunofluorescent analysis of HeLa cells using 14896-1-AP (RRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Milzgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14896-1-AP bindet in WB, IF/ICC, ELISA RRP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRP1 fusion protein Ag6686
• Vollständiger Name: ribosomal RNA processing 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC000380
• Gene symbol: RRP1
• Gene ID (NCBI): 8568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Pre-ribosomal WDR74 module coordinates the early and late pre-rRNA processing stages for the NVL2-mediated regulation of 60S ribosome biogenesis; Authors - Yuya Hirooka