RRP1B Polyklonaler Antikörper

RRP1B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27723-1-AP

Synonyme

RRP1-like protein B, RRP1 like protein B, Ribosomal RNA processing protein 1 homolog B

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  WB analysis of HEK-293 using 27723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27723-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27723-1-AP (RRP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27723-1-AP (RRP1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 27723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 27723-1-AP (RRP1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27723-1-AP bindet in WB, IHC, IF/ICC, ELISA RRP1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRP1B fusion protein Ag23405
• Vollständiger Name: ribosomal RNA processing 1 homolog B (S. cerevisiae)
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC028386
• Gene symbol: RRP1B
• Gene ID (NCBI): 23076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal RNA Processing 1 Homolog B (RRP1B) is a protein that has been implicated in several biological processes, particularly in the context of cancer progression and viral replication. RRP1B was first identified as a metastasis susceptibility gene in breast cancer through its ability to modulate gene expression in a manner that can be used to accurately predict prognosis in breast cancer . It has been shown to interact with the metastasis modifier gene, Sipa1, and modulate elements of metastasis predictive gene expression signatures.