RSK2 Polyklonaler Antikörper

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• KD/KO Validated

RSK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23762-1-PBS

Synonyme

RPS6KA3, ISPK1, ISPK 1, Insulin-stimulated protein kinase 1, HU 3

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  WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis using 23762-1-AP (same clone as 23762-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 23762-1-AP (same clone as 23762-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 23762-1-AP (same clone as 23762-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23762-1-AP (same clone as 23762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23762-1-AP (RSK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 23762-1-AP (same clone as 23762-1-PBS)

  Immunofluorescent analysis of HUVEC cells using 23762-1-AP (RSK2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23762-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA RSK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSK2 fusion protein Ag20620
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Berechnetes Molekulargewicht: 740 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC096301
• Gene symbol: RSK2
• Gene ID (NCBI): 6197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.