RSK4 Polyklonaler Antikörper

RSK4 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21877-1-AP

Synonyme

RPS6KA6, p90RSK6, pp90RSK4, S6K alpha 6

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  WB analysis using 21877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21877-1-AP (RSK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-12 using 21877-1-AP

  IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with PC-12 cells lysate 1560 ug.

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  IP experiment of MCF-7 using 21877-1-AP

  IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with MCF-7 cells lysate 1520 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: MCF-7-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21877-1-AP bindet in WB, IP, ELISA RSK4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSK4 fusion protein Ag16299
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 6
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC148802
• Gene symbol: RPS6KA6
• Gene ID (NCBI): 27330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. RSK4 is a predominantly cytosolic protein with very low expression and several characteristics of the RSK family kinases, including the presence of two functional kinase domains and a C-terminal docking site for ERK (PMID: 39114716, 30579335). Endogenous RSK4 showed the same level of phosphorylation of Ser232, Ser372, and Ser389 in starved and growth factor-stimulated cells, and mutational analysis demonstrated that these sites are important in stimulation of kinase activity (PMID: 15632195).