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RSK4 Polyklonaler Antikörper

RSK4 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21877-1-PBS

Synonyme

RPS6KA6, p90RSK6, pp90RSK4, S6K alpha 6

Filter:
  • Western Blot (WB) analysis of various lysates using RSK4 Polyclonal antibody (21877-1-AP)

    WB analysis using 21877-1-AP (same clone as 21877-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21877-1-AP (RSK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21877-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-12 cells using RSK4 Polyclonal antibody (21877-1-AP)

    IP experiment of PC-12 using 21877-1-AP (same clone as 21877-1-PBS)

    IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with PC-12 cells lysate 1560 ug. This data was developed using the same antibody clone with 21877-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RSK4 Polyclonal antibody (21877-1-AP)

    IP experiment of MCF-7 using 21877-1-AP (same clone as 21877-1-PBS)

    IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with MCF-7 cells lysate 1520 ug. This data was developed using the same antibody clone with 21877-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21877-1-PBS bindet in WB, IP, Indirect ELISA RSK4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RSK4 fusion protein Ag16299
Vollständiger Name ribosomal protein S6 kinase, 90kDa, polypeptide 6
Berechnetes Molekulargewicht 745 aa, 84 kDa
Beobachtetes Molekulargewicht84 kDa
GenBank-ZugangsnummerBC148802
Gene symbol RPS6KA6
Gene ID (NCBI) 27330
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. RSK4 is a predominantly cytosolic protein with very low expression and several characteristics of the RSK family kinases, including the presence of two functional kinase domains and a C-terminal docking site for ERK (PMID: 39114716, 30579335). Endogenous RSK4 showed the same level of phosphorylation of Ser232, Ser372, and Ser389 in starved and growth factor-stimulated cells, and mutational analysis demonstrated that these sites are important in stimulation of kinase activity (PMID: 15632195).