RSPH9 Polyklonaler Antikörper
RSPH9 Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 23253-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe | 
| Erfolgreiche Detektion in IHC | Rattenhirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 6 publications below | 
| IF | See 7 publications below | 
Produktinformation
23253-1-AP bindet in WB, IHC, IF, ELISA RSPH9 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Maus | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | RSPH9 fusion protein Ag19745 | 
| Vollständiger Name | radial spoke head 9 homolog (Chlamydomonas) | 
| Berechnetes Molekulargewicht | 276 aa, 31 kDa | 
| Beobachtetes Molekulargewicht | 31-35 kDa | 
| GenBank-Zugangsnummer | BC029519 | 
| Gene symbol | RSPH9 | 
| Gene ID (NCBI) | 221421 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-affinitätsgereinigt | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
RSPH9 (Radial Spoke Head 9 Homolog) is a protein involved in the structure and function of cilia and flagella. Cilia and flagella are hair-like structures that extend from the surface of many eukaryotic cells and play critical roles in cell motility, sensory perception, and signaling. The RSPH9 protein is a component of the radial spoke, a structure within the axoneme (the core of cilia and flagella) that is essential for the regulation of ciliary and flagellar movement.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for RSPH9 antibody 23253-1-AP | Protokoll herunterladen | 
| IHC protocol for RSPH9 antibody 23253-1-AP | Protokoll herunterladenl | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Elife LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility | ||
| Hum Reprod CALR3 defects disrupt sperm-zona pellucida binding in humans: new insights into male factor fertilization failure and relevant clinical therapeutic approaches | ||
| Hum Mol Genet Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse. | ||
| Eur J Hum Genet Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility. | ||
| Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
| EBioMedicine CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Célia (Verified Customer) (10-28-2024) | I tested 1/100 to be sure that it works in IF but the concentration was a little high so 1/200 should still be fine. 
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