RSPH9 Polyklonaler Antikörper

RSPH9 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 23253-1-AP

Synonyme

MRPS18AL1, CILD12, C6orf206

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• 

  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23253-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 23253-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 23253-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 23253-1-AP (RSPH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 7 publications below

Produktinformation

23253-1-AP bindet in WB, IHC, IF, ELISA RSPH9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSPH9 fusion protein Ag19745
• Vollständiger Name: radial spoke head 9 homolog (Chlamydomonas)
• Berechnetes Molekulargewicht: 276 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31-35 kDa
• GenBank-Zugangsnummer: BC029519
• Gene symbol: RSPH9
• Gene ID (NCBI): 221421
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RSPH9 (Radial Spoke Head 9 Homolog) is a protein involved in the structure and function of cilia and flagella. Cilia and flagella are hair-like structures that extend from the surface of many eukaryotic cells and play critical roles in cell motility, sensory perception, and signaling. The RSPH9 protein is a component of the radial spoke, a structure within the axoneme (the core of cilia and flagella) that is essential for the regulation of ciliary and flagellar movement.

Publikationen

Species	Application	Title
mouse	WB	Elife; LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility; Authors - Jae Yeon Hwang
human	IF	Hum Reprod; CALR3 defects disrupt sperm-zona pellucida binding in humans: new insights into male factor fertilization failure and relevant clinical therapeutic approaches; Authors - Yang Gao
human	WB, IF	Hum Mol Genet; Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse.; Authors - Jintao Zhang
		Eur J Hum Genet; Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility.; Authors - Shushu Zhou
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	EBioMedicine; CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men; Authors - Hui-Juan Jin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Célia (Verified Customer) (10-28-2024)

I tested 1/100 to be sure that it works in IF but the concentration was a little high so 1/200 should still be fine.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Spermatozoa