RTF1 Polyklonaler Antikörper

RTF1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12170-1-PBS

Synonyme

---

• 

  WB analysis of MCF-7 using 12170-1-AP (same clone as 12170-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 12170-1-AP (same clone as 12170-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12170-1-AP (same clone as 12170-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 12170-1-AP (same clone as 12170-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12170-1-AP (same clone as 12170-1-PBS)

  IP result of anti-RTF1 (IP:12170-1-AP, 4ug; Detection:12170-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12170-1-AP (same clone as 12170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12170-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12170-1-PBS bindet in WB, IHC, IP, Indirect ELISA RTF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTF1 fusion protein Ag2812
• Vollständiger Name: Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 585aa,67 kDa; 710aa,80 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC015052
• Gene symbol: RTF1
• Gene ID (NCBI): 23168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.