RTKN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RTKN2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17458-1-AP

Synonyme

PH domain-containing family K member 1, Pleckstrin homology domain-containing family K member 1, PLEKHK1, rhotekin 2, Rhotekin-2

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  WB analysis of HL-60 using 17458-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17458-1-AP

  WB result of RTKN2 antibody (17458-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RTKN2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 17458-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17458-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lymph using 17458-1-AP

  mouse lymph tissue were subjected to SDS PAGE followed by western blot with 17458-1-AP (RTKN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17458-1-AP (RTKN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17458-1-AP (RTKN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 17458-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17458-1-AP (RTKN2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 17458-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RTKN2 antibody (17458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HEK-293-Zellen, Maus-Lymphgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

17458-1-AP bindet in WB, IHC, IF/ICC, ELISA RTKN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTKN2 fusion protein Ag11098
• Vollständiger Name: rhotekin 2
• Berechnetes Molekulargewicht: 163aa,19 kDa; 609aa,69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC025765
• Gene symbol: RTKN2
• Gene ID (NCBI): 219790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Rep; Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity.; Authors - Brendan Gongol ; KD Validated
human	WB,IHC	J Viral Hepat; Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046-miR-1299-RTKN2 ceRNA pathway; Authors - Li Zhang
human	WB	Sci Rep; RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway; Authors - Xiaomei Zhang