RTN1 (Isoform RTN1-C) Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RTN1 (Isoform RTN1-C) Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15048-1-AP

Synonyme

NSP, NSP-C, reticulon 1, RTN1, RTN1 (Isoform RTN1-C), RTN1-C

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Galerie der Validierungsdaten

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  WB analysis using 15048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15048-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15048-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Hirngewebe, humanes Plazenta-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

15048-1-AP bindet in WB, IHC, IF, ELISA RTN1 (Isoform RTN1-C) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTN1 (Isoform RTN1-C) fusion protein Ag7031
• Vollständiger Name: reticulon 1
• Berechnetes Molekulargewicht: 84 kDa, 39 kDa, 24 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000314
• Gene symbol: RTN1
• Gene ID (NCBI): 6252
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN1 (also known as NSP) gene is expressed predominantly in neuroendocrine tissues, and produces three isoforms termed RTN1-A (NSP-A), RTN1-B (NSP-B), and RTN1-C (NSP-C). This antibody is raised against the full-length protein of human RTN1-C.

Publikationen

Species	Application	Title
mouse, rat	WB, IF	Cell Death Dis; RTN1-C mediates cerebral ischemia/reperfusion injury via ER stress and mitochondria-associated apoptosis pathways.; Authors - Lingli Gong ; KD Validated
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu
rat,mouse	WB	Acta Biochim Biophys Sin (Shanghai); RTN1-C mediates cerebral ischemia/reperfusion injury via modulating autophagy.; Authors - Jun Ling ; KD Validated
mouse	WB	NPJ Parkinsons Dis; Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities; Authors - Jia Yu
mouse	WB	Brain Res Bull; RTN1-C is involved in high glucose-aggravated neuronal cell subjected to oxygen-glucose deprivation and reoxygenation injury via endoplasmic reticulum stress.; Authors - Muya Lin