RTN2 Rekombinanter Antikörper

RTN2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250823C1

Kat-Nr. : 86162-1-RR

Synonyme

Neuroendocrine-specific protein-like 1, Neuroendocrine-specific protein-like I, NSPL1, NSPLI, NSP-like protein 1

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  WB analysis using 86162-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86162-1-RR

  Various cell lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 86162-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86162-1-RR (RTN2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 86162-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86162-1-RR (RTN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86162-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86162-1-RR against Human RTN2 were performed. The affinity constant is 0.358 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, K-562-Zellen, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86162-1-RR bindet in WB, IHC, ELISA RTN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RTN2 fusion protein Ag1655
• Vollständiger Name: reticulon 2
• Berechnetes Molekulargewicht: 59 kDa, 51 kDa, 22 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC014244
• Gene symbol: RTN2
• Gene ID (NCBI): 6253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.