RTN3 Polyklonaler Antikörper

RTN3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 12055-2-AP

Synonyme

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

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Galerie der Validierungsdaten

• 

  WB analysis using 12055-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12055-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12055-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12055-2-AP

  IP Result of anti-RTN3 (IP:12055-2-AP, 4ug; Detection:12055-2-AP 1:400) with HeLa cells lysate 2000ug.

• 

  IHC staining of human gliomas using 12055-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12055-2-AP (RTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12055-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12055-2-AP (RTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, U2OS-Zellen, U-87 MG-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

12055-2-AP bindet in WB, IP, IHC, IF, ELISA RTN3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTN3 fusion protein Ag2685
• Vollständiger Name: reticulon 3
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 24 kDa, 100-113 kDa
• GenBank-Zugangsnummer: BC011394
• Gene symbol: RTN3
• Gene ID (NCBI): 10313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.

Publikationen

Species	Application	Title
human	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
		Acta Neuropathol; Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS; Authors - Amr Aly
rat	WB	J Pineal Res; Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression; Authors - Min Yao
human	WB	Dev Cell; TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules.; Authors - Kimya Nourbakhsh
human	WB,IF,IP	Acta Neuropathol Commun; Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia.; Authors - Dwayne J Byrne
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This is a very good antibody for immunoblotting.

• Applications: Western Blot
• Cell Tissue Type: HeLa

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