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RTN3 Polyklonaler Antikörper

RTN3 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12055-2-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using RTN3 Polyclonal antibody (12055-2-AP)

    WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RTN3 Polyclonal antibody (12055-2-AP)

    WB analysis of human brain using 12055-2-AP (same clone as 12055-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RTN3 Polyclonal antibody (12055-2-AP)

    WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RTN3 Polyclonal antibody (12055-2-AP)

    IP experiment of HeLa using 12055-2-AP (same clone as 12055-2-PBS)

    IP result of anti-RTN3 (IP:12055-2-AP, 4ug; Detection:12055-2-AP 1:400) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12055-2-PBS bindet in WB, IP, Indirect ELISA RTN3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RTN3 fusion protein Ag2685
Vollständiger Name reticulon 3
Berechnetes Molekulargewicht 113 kDa
Beobachtetes Molekulargewicht 24 kDa, 100-113 kDa
GenBank-ZugangsnummerBC011394
Gene symbol RTN3
Gene ID (NCBI) 10313
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.