RTN4/NOGO Polyklonaler Antikörper

RTN4/NOGO Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 10740-1-AP

Synonyme

ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, RTN4/NOGO, SP1507

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Galerie der Validierungsdaten

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  WB analysis using 10740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 10740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10740-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:100 (under 10x lens).

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  FC experiment of SH-SY5Y using 10740-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug RTN4/NOGO antibody (10740-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10740-1-AP bindet in WB, IP, IHC, IF, FC, ELISA RTN4/NOGO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTN4/NOGO fusion protein Ag1164
• Vollständiger Name: reticulon 4
• Berechnetes Molekulargewicht: 130 kDa
• Beobachtetes Molekulargewicht: 190-210 kDa, 45 kDa, 22-25 kDa
• GenBank-Zugangsnummer: BC007109
• Gene symbol: RTN4
• Gene ID (NCBI): 57142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. Some isoforms of RTN4 have been described. RTN4A (Nogo-A, runs at ~200 kDa), RTN4B (Nogo-B, 40-55 kDa), and RTN4C (Nogo-C, 22-25 kDa) are three major isoforms (PMID: 31092426; 16469703).

Publikationen

Species	Application	Title
human	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human,mouse	WB,IF	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Autophagy; DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.; Authors - Xiaoyu Tian
human	WB	Autophagy; SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.; Authors - Xiaolin Zhang
human	WB,IF	Acta Neuropathol Commun; Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia.; Authors - Dwayne J Byrne