RTN4IP1 Polyklonaler Antikörper

RTN4IP1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15636-1-AP

Synonyme

NIMP, NOGO-interacting mitochondrial protein, Reticulon-4-interacting protein 1, mitochondrial

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  WB analysis using 15636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15636-1-AP (RTN4IP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 15636-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15636-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15636-1-AP bindet in WB, IF/ICC, ELISA RTN4IP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTN4IP1 fusion protein Ag8100
• Vollständiger Name: reticulon 4 interacting protein 1
• Berechnetes Molekulargewicht: 396 aa, 44 kDa
• Beobachtetes Molekulargewicht: 32-44 kDa, 24 kDa
• GenBank-Zugangsnummer: BC006399
• Gene symbol: RTN4IP1
• Gene ID (NCBI): 84816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RTN4IP1 (Reticulon-4-interacting protein 1, mitochondrial), also known as NIMP. It colocalizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria (PMID: 26593267), which is widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta (PMID: 12067236). The protein plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury. The protein has three isoforms with molecular weights of 43 kDa, 32 kDa and 24 kDa respectively.