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RTN4IP1 Polyklonaler Antikörper

RTN4IP1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15636-1-PBS

Synonyme

NIMP, NOGO-interacting mitochondrial protein, Reticulon-4-interacting protein 1, mitochondrial

Filter:
  • Western Blot (WB) analysis of various lysates using RTN4IP1 Polyclonal antibody (15636-1-AP)

    WB analysis using 15636-1-AP (same clone as 15636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15636-1-AP (RTN4IP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15636-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RTN4IP1 Polyclonal antibody (15636-1-AP)

    IF Staining of HepG2 using 15636-1-AP (same clone as 15636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15636-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RTN4IP1 Polyclonal antibody (15636-1-AP)

    IF Staining of HepG2 using 15636-1-AP (same clone as 15636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 15636-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15636-1-PBS bindet in WB, IF/ICC, Indirect ELISA RTN4IP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RTN4IP1 fusion protein Ag8100
Vollständiger Name reticulon 4 interacting protein 1
Berechnetes Molekulargewicht 396 aa, 44 kDa
Beobachtetes Molekulargewicht32-44 kDa, 24 kDa
GenBank-ZugangsnummerBC006399
Gene symbol RTN4IP1
Gene ID (NCBI) 84816
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RTN4IP1 (Reticulon-4-interacting protein 1, mitochondrial), also known as NIMP. It colocalizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria (PMID: 26593267), which is widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta (PMID: 12067236). The protein plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury. The protein has three isoforms with molecular weights of 43 kDa, 32 kDa and 24 kDa respectively.