RUFY1 Rekombinanter Antikörper

RUFY1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250159A3

Kat-Nr. : 85839-4-PBS

Synonyme

FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, RABIP4, RUFY 1

---

• 

  WB analysis using 85839-4-RR (same clone as 85839-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85839-4-RR (RUFY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85839-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 85839-4-RR (same clone as 85839-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RUFY1 antibody (85839-4-RR, Clone: 250159A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85839-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85839-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85839-4-RR against Human RUFY1 were performed. The affinity constant is 0.444 nM.

---

Geprüfte Anwendungen

Produktinformation

85839-4-PBS bindet in WB, IF/ICC, Indirect ELISA RUFY1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RUFY1 fusion protein Ag4324
• Vollständiger Name: RUN and FYVE domain containing 1
• Berechnetes Molekulargewicht: 708 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 69 kDa
• GenBank-Zugangsnummer: BC032571
• Gene symbol: RUFY1
• Gene ID (NCBI): 80230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.